| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Redoubt Soccer Association - 01 2009 Redoubt Generals |
0312-013B5U-0009 | Richard Tate |
| A2 | Jackson Soccer Club JSC 2009 Boys Gold |
0609-012B5U-0012 | Malang Jarju |
| A3 | Nashville United Soccer Academy - 01 NUSA B09 Navy |
0534-012B5U-0022 | Thomas Campbell |
| A4 | Johnson City Futbol Club - 01 JCFC 2009B |
0216-012B5U-0129 | Dan Trull |
| A5 | Soccer Ole OLE FC 09B Premier |
0632-022B5U-0014 | Evan Hacker |
| A6 | Tennessee Soccer Club TSC Murfreesboro 2009 Boys Showcase |
0522-012B5U-2204 | Enrique Suarez |
| A7 | Chattanooga FC Academy Chattanooga FC - 2009B Navy |
0316-022B5U-7931 | Benjamin Wilkinson |
| A8 | Lobos Rush (Collierville Soccer Association) 2009 Lobos Rush E64 |
0603-012B5U-0052 | Christopher Wright |