| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Lobos Rush (Collierville Soccer Association) 2009 Lobos Rush ECNL |
0603-011B5U-0053 | Sylvanus Johnson |
| A2 | Stones River Futbol Club - 01 Stones River FC 2009 Black |
0521-011B5U-0015 | Cesar Salinas Sr |
| A3 | Germantown Legends Germantown Legends 09 Black |
0606-011B5U-0008 | Carey Talley |
| A4 | Nashville United Soccer Academy - 01 NUSA B09 Maroon |
0534-011B5U-0009 | Jared MacLane |
| A5 | Pateadores Soccer Club - Nashville Pats 2009 Blue |
0500-01D1B5U-0003 | Maria Jose Harispuru |
| A6 | Stones River Futbol Club - 01 Stones River FC 2009 Gray |
0521-011B5U-0014 | Bernardo Ortega |
| A7 | One Knoxville One Knox 09B- NPL |
0293-01D1B5U-0009 | Jordan Bell |
| A8 | All-In FC TN All-In FC TN 09B Chippewas |
0443-01SLB5U-0007 | Davis Draper |