| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Tennessee United Soccer Club TUSC 2008 Boys Red |
0424-012B6U-0019 | Benjamin Walker |
| A2 | Soccer Ole OLE FC 08B Premier |
0632-021B6U-0015 | Sasa Kostic |
| A3 | FC Alliance Select Travel Teams FC Alliance 2008B RL |
0204-012B6U-0265 | Edgard Semaan |
| A4 | Lobos Rush (Collierville Soccer Association) 2008 Lobos Rush E64 |
0603-012B6U-0023 | Isaac Johnson |
| A5 | Germantown Legends Germantown Legends 08 White |
0606-012B6U-0023 | Kirk Dunaway |
| A6 | Johnson City Futbol Club - 01 JCFC 2008B Black |
0216-012B6U-0002 | Neal Lewis |
| A7 | Chattanooga FC Academy Chattanooga FC - 2008B Navy |
0316-022B6U-7933 | Gary Smith |
| A8 | Aris FC Aris FC - 2008 Boys Gold |
0530-022B6U-0152 | William Thorne |