| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Lobos Rush (Collierville Soccer Association) 2007 Lobos Rush ECNL |
0603-011B7U-0057 | Sylvanus Johnson |
| A2 | Germantown Legends Germantown Legends 07 Premier |
0606-011B7U-0011 | Justin Rose |
| A3 | One Knoxville One Knox 07B- NPL |
0293-01D1B7U-0025 | Nicolas Jaramillo |
| A4 | Nashville United Soccer Academy - 01 NUSA B07 Maroon |
0534-011B7U-0011 | Adam Harris |
| A5 | Tennessee Soccer Club TSC Williamson 2007 Boys Showcase |
0522-012B7U-0065 | James Pollock |
| A6 | Tennessee United Soccer Club TUSC 2007 Boys NPL |
0424-011B7U-0010 | Juan Holguin |
| A7 | Tennessee Soccer Club TSC Sumner 2007 Boys Showcase |
0522-011B7U-2194 | Travis Pulley |
| A8 | Tennessee Soccer Club TSC Murfreesboro 2007 Boys Showcase |
0522-011B7U-2192 | Enrique Suarez |