| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Knoxville United Soccer Association - 01 KUSA 06B Stars |
0210-012B8U-0001 | Francis Colbert |
| A2 | Nashville United Soccer Academy - 01 NUSA B06 Navy |
0534-012B8U-0025 | Jared MacLane |
| A3 | Chattanooga FC Academy CSM Forge U18 Boys |
0316-022B8U-7959 | Matthew Jelley |
| A4 | Jackson Soccer Club JSC 2006 BOYS |
0609-012B8U-0005 | Christian Young |
| A5 | Tennessee United Soccer Club TUSC 2006 Boys Red |
0424-012B8U-0021 | Cabel Ross |
| A6 | Columbia Soccer Association - 01 2006 Columbia Arsenal |
0504-012B8U-0001 | Taylor Freels |
| A7 | Greater Cleveland Soccer Association Flames 2006 Maroon |
0306-01CB8U-8949 | Leo Arntz |
| A8 | Camp Forest Football Club Camp Forest FC 2006 Boys |
0523-022B8U-0002 | Andrew Sain |