| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Lobos Rush (Collierville Soccer Association) U19 Lobos Rush ECNL |
0603-011B9U-0054 | Clint Browne |
| A2 | Nashville United Soccer Academy - 01 NUSA B05 Maroon |
0534-011B9U-0013 | Quincy Thomas |
| A3 | Play Where You Stay - 01 PWYS '05 Boys |
0653-011B9U-2011 | Alvaro Martinez Guija |
| A4 | Stones River Futbol Club - 01 Stones River FC 2005 Black |
0521-011B9U-0025 | Bouttavong Thompson |
| A5 | One Knoxville One Knox 06/05B- NPL |
0293-01D1B9U-0012 | Nicolas Jaramillo |
| A6 | Jackson Soccer Club JSC 2005 Boys |
0609-012B9U-0004 | Malang Jarju |
| A7 | ALBION SC Nashville (Formerly Southern Premier Soccer) ALBION SC Nashville - B05/06 Academy Elite |
0540-02D1B9U-0039 | Brent Goulet |
| A8 | Germantown Legends Germantown Legends 05/06 White |
0606-011B9U-0012 | Antonio Carbognani |